9 research outputs found
Wavelet Signal Processing of Physiologic Waveforms
The prime objective of this piece of work is to devise novel techniques for computer
based classification of Electrocardiogram (ECG) arrhythmias with a focus on less computational time and better accuracy. As an initial stride in this direction, ECG beat classification is achieved by using feature extracting techniques to make a neural network (NN) system more effective. The feature extraction technique used is Wavelet Signal Processing. Coefficients from the discrete wavelet transform were used to represent the ECG diagnostic information and features were extracted using the coefficients and were normalised. These feature sets were then used in the classifier i.e. a simple feed forward back propagation neural network (FFBNN). This paper presents a detail study of the classification accuracy of ECG signal by using these four structures for computationally efficient early diagnosis. Neural network used in this study is a well-known neural network architecture named as multi-Layered perceptron (MLP) with back propagation training algorithm. The ECG signals have been taken from MIT-BIH ECG database, and are used in training to classify 3 different Arrhythmias out of ten arrhythmias. These are normal sinus rhythm, paced beat, left bundle branch block. Before testing, the proposed structures are trained by back propagation algorithm. The results show that the wavelet decomposition method is very effective and efficient for fast computation of ECG signal analysis in conjunction with the classifier
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Kanamycin-Mediated Conformational Dynamics of Escherichia coli Outer Membrane Protein TolC.
TolC is a member of the outer membrane efflux proteins (OEPs) family and acts as an exit duct to export proteins, antibiotics, and substrate molecules across the Escherichia coli cell membrane. Export of these molecules is evidenced to be brought about through the reversible interactions and binding of substrate-specific drug molecules or antibiotics with TolC and by being open for transport, which afterward leads to cross-resistance. Hence, the binding of kanamycin with TolC was monitored through molecular docking (MD), the structural fluctuations and conformational changes to the atomic level. The results were further supported from the steady-state fluorescence binding and isothermal titration calorimetry (ITC) studies. Binding of kanamycin with TolC resulted in a concentration dependent fluorescence intensity quenching with 7 nm blue shift. ITC binding data maintains a single binding site endothermic energetic curve with binding parameters indicating an entropy driven binding process. The confirmational changes resulting from this binding were monitored by a circular dichroism (CD) study, and the results showed insignificant changes in the α-helix and β-sheets secondary structure contents, but the tertiary structure shows inclusive changes in the presence of kanamycin. The experimental data substaintially correlates the RMSD, R g, and RMSF results. The resulting conformational changes of the TolC-kanamycin complexation was stabilized through H-bonding and other interactions
Evaluation of 8-Hydroxy deoxyguanosine levels, as a marker of oxidative stress in chronic gingivitis and chronic periodontitis individuals during the course of phase 1 periodontal therapy in comparison with periodontally healthy individuals
Aim: The aim was to evaluate the levels of 8-Hydroxy deoxyguanosine levels, as a marker of oxidative stress in Chronic Gingivitis and Chronic Periodontitis individuals during the course of Phase 1 periodontal therapy in comparison with Periodontally healthy Individuals. Materials and Method: All the individuals were divided into 3 groups each including 16 subjects. Group 1 included healthy subjects and group 3 and 4 included subjects with chronic gingivitis and chronic periodontitis respectively. Saliva samples were collected and the 8-OHdG levels were assessed. Results: The levels reduced to 1.59±0.50 in chronic periodontitis group one month after scaling and root planing. A statistically non-significant value of 0.09 was recorded. The levels in chronic gingivitis group were 1.20±0.89. Conclusion: From the above study it can be concluded that 8-OHdG is an important biomarker of oxidative stress and is increased in patients with chronic gingivitis and chronic periodontitis
Implant retained overdenture using OT equator attachments: A case series
The aim of this clinical report is to demonstrate the technique used with OT‑Equator attachment with chair side incorporation of housings in pre-fabricated denture. Implant retained overdentures are evolving as an optimum and cost-effective treatment modality for patients with edentulism and the evolution of attachments have led to their increased use. The Article Describes a Series of Cases where implant retained overdentures dentures were given to the patient using OT EQUATOR attachments to improve the retentive properties of the dentures thus elevating their chewing efficiency and also the quality of speaking and boosting the overall confidence of the patient. The treatment described in the article should be 1advocated and used wherever possible as it preserves both hard and soft tissues. The OT EQUATOR attachment is specially indicated in cases of compromised inter-arch space and where retention is of utmost priority as described in this case report. The advantage of the attachment is that it is very easy and convenient to use and also offers a chair-side fabricating process
Fiber-Reinforced Composite-Fixed Partial Denture: A Case Series
In most societies, lack of anterior teeth is a serious problem in a patient’s social life. While traditional removable partial dentures and implant support restorations are often the treatment of choice, fiber-reinforced composites are a conservative, fast, and cost-effective alternative for single and multiple tooth replacement